Virion
Simian virus 40
This cartoon depicting SV40 was created by Stefan A. Welte, University of Tübingen using ProteinExplorer (Martz 2002) (www.proteinexplorer.org) and the PDB code 1sva (Liddington et al 1991)
Genome
Genome organization of polyomavirus simian virus 40, strain 776
The circle represents the circular SV40 DNA genome. Nucleotide numbers begin and end at the origin (Ori) of viral DNA replication (0/5243). Unique Bgl-1 and Sfi-1 sites flank the Ori. A unique EcoRI site is shown at map unit 0/1. Boxed arrows indicate the open reading frames that encode the viral proteins. Arrowheads point in the direction of transcription; the beginning and end of each open reading frame is indicated by nucleotide numbers. The regulatory region is shown at the top; Ori, origin of DNA replication; 21-bp repeats, GC-rich SP1 binding sites and location of the SES; 72-bp, tandemly repeated 72-bp sequences within the enhancer. Numbers above the diagram identify nucleotide positions that border specific regulatory region segments.
Replication
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History
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Genus Members
Nucleotide Sequences
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| Genomic Region | Virus Species / Strain | Nucleotides | Accession Number | Reference | |
|---|---|---|---|---|---|
| Complete genome | SV40, 776 | 5,243 | J02400.1 | Fiers W et al (1978) Nature 273:113-120; Reddy VB et al (1978) Science 200:494-502 | |
Proteins
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| Protein Name | Abbreviation | Amino Acids | Molecular Weight (kDa) | Time of Expression | Accession Number | Additional Information | |
|---|---|---|---|---|---|---|---|
| Agnoprotein | agno; LP1 | 62 | 7.9 | Late | AAB59920.1 | Facilitates virus assembly | |
| Large tumor antigen | T-ag | 708 | 90 | Early | AAB59924.1 | Major transforming protein; regulates viral transcription; binds key host cell regulators p53 and pRB; initiates viral DNA unwinding and replication | |
| Small tumor antigen | t-ag | 174 | 20 | Early | AAB59925.1 | Promotes cell transformation by negative regulation of the protein phosphatase 2A family of phosphatases | |
| Tiny tumor antigen | 17kT | 135 | 17 | Early | Regulates pRB proteins | ||
| Viral coat protein 1 | VP1 | 364 | 45 | Late | AAB59923.1 | Major structural protein; receptor binding protein | |
| Viral coat protein 2 | VP2 | 352 | 42 | Late | AAB59921.1 | Minor structural protein | |
| Viral coat protein 3 | VP3 | 234 | 30 | Late | AAB59922.1 | Minor structural protein | |
| Viral coat protein 4 | VP4 | 125 | 15 | Very late | Nonstructural protein; induces lysis of infected cells | ||
Biology
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Diseases
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Diagnosis
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| Diagnosis Method | Virus Species / Strain | Sample Material | Detection Target | Reference | |
|---|---|---|---|---|---|
Vaccine Strains
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| Vaccine Name | Backbone Virus Species / Strain | Attenuation Procedure | Additional Information | Reference | |
|---|---|---|---|---|---|
Vectors
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| Vector Name | Backbone Virus Species / Strain | Application | Insertion Capacity (kb) | Additional Information | Reference | |
|---|---|---|---|---|---|---|
| pGL4.13(luc2/SV40) | Regulatory region, SV40 strain 776 in ColEI plasmid replication origin | Internal control | N/A | Ampicillin resistance; reporter expressing firefly luciferase protein | Yamasaki K et al (2009) Invest Ophthalmol Vis Sci 50:604-613 | |
| pRL-SV40 | Regulatory region, SV40 strain 776 in ColEI plasmid replication origin | Internal control | N/A | Ampicillin resistance; reporter expressing Renilla reniformis protein | Zhang Y et al (2007) Proc Natl Acad Sci USA 104:10613-10618 | |